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Proceedings of Past Forest Nursery Association Meetings:

Protocols for Mass Micropropagation of Antelope and Desert Bitterbrush
Annette Leege-Brusven, John L. Edson, David L. Wenny and Min Hironaka
1994: Moscow, ID
Vegetative propagation of Purshia tridentata and P. glandulosa could potentially capture and multiply valuable genetic traits such as fire resistance and produce plants which reproduce in a shorter time than seedlings. The goal of this research was to develop mass propagation protocols for antelope and desert bitterbrush using micropropagation techniques. Microshoot tips were incubated in vitro on alternative nutrient media supplemented with different levels of cytokinin and auxin growth regulators. An average of 7.9 shoots/explant of P. tridentata were produced after 1 month on Woody Plant Medium (WPM) supplemented with 0.1 mg/l benzlyadenine (BA). P. glandulosa multiplied 5-fold over 1 month on the same media. Best rooting occurred ex vitro for both species. P. tridentata microshoots rooted at 88.6% after treatment with Hormex(R) #1 (0.1% IBA) and P. glandulosa microshoots rooted up to 78% after treatment with Hormex(R) #3 (0.3% IBA). Flowering and fruiting of micropropagated plantlets from both species occurred after one season's growth in the greenhouse. These successful mass propagation systems for bitterbrush could play an important role in range restoration.
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